Construction of Tissue Culture and Genetic Transformation Systems of Nicotiana benthamiana

[Objective]The research aimed to obtain the transfer of glucagon likepeptide-1 gene Nicotiana benthamiana plants.[Method] With sterile tobacco leaf explants as the experimental material,the regeneration system of tobacco was established and transgenic Nicotiana benthamiana was obtained through Agrobacterium infection.[Result]By multiple comparison and repeated experiments,the optimal Nicotiana benthamiana differentiation medium of MS+1.5 mg/L 6-BA +0.1 mg/L NAA was determined,so did the rooting medium MS +0.1 mg/L IBA.By kanamycin selection pressure gradient experiments,the selection concentration was identified as 5 mg/L kanamycin,later disseminated by Agrobacterium leaf disc method for genetic transformation.GUS acquired resistance to staining the buds and the positive signal was obtained.[Conclusion]Gene GLP-1 was integrated into the genome of tobacco Nicotiana benthamiana.