Development of A PCR Method for Rapid Detection of Bacillus Licheniformis Based on gyrB Gene

In this study, based on gyrB gene sequences from Bacillus licheniformis, a pair of specific primers was designed. The size of the amplified product were 507 bp. The amplified results from Bacillus DNA were positive, but the results were negative from control strain. The sensitivity for Bacillus licheniformis was 1pg DNA. A PCR method for detection of Bacillus licheniformis was established. The method had the advantages of high-efficiency,high-sensitivity and high-specificity, and which set a good foundation for isolating Bacillus licheniformis from a complex microbial community samples.