Transition of congo-red in cellulose congo-red liquid medium was studied by cellulase-producing fungi. Results showed that cellulose was decomposed and turned into polysaccharides by cellulase-producing fungi,which possessed of glucans structure,then polysaccharides bonded congo-red to form polysaccharides-congo red complex. The complex was not only adhered on the surface of hyphae,but also absorbed inside them. With that,congo-red deposited in hyphae,and colored the colony red. So,cong-red medium can be used as a special medium to identify fungi possessing cellulase.