The pot experiment was carried out with Eum-Orthic Anthrosols to study the responses of winter wheat in Assimilative matter and nitrogen translocation before anthesis to nitrogen fertilizer using 6 cultivars(NR9405,9430,Yanshi 9,Xiaoyan6,Shaan229 and Xinong2208) as material under two treatments of nitrogen fertilizer(0 and 0.02 g nitrogen per kilogram soil).The results showed that the translocation amount,translocation efficiency,and proportion of dry matter translocation in grain before anthesis,grain yield,N uptake amount before and after anthesis,and total N uptake amount were significantly increased by applying N fertilizer,but the translocation efficiency and contribution rate to grain from nitrogen before anthesis,and nitrogen use efficiency reduced.All the measured indexes existed significantly differences between varieties.After applying nitrogen fertilizer,the increased among of variety in all the measured indexes was also apparently different from that of others.This indicated that the response of winter wheat for N fertilizer was significantly different among varieties in all the measured indexes.Therefore,the translocation of assimilative matter and N before anthesis to grain could be promoted to increase grain yield and nitrogen use efficiency of winter wheat by variety selection and nitrogen fertilizer application.