Comparison of Methods to Prepare Genomic DNA from Baccilus fastidious

Objectives:To compare methods for the preparation of genomic DNA from Bacillus fastidious.Methods: Genomic DNA was extracted by classical CTAB method,modified CTAB method with the use of lysozyme and UniQ ion-exchange method,the quality and quantity of genomic DNA were compared.Results: The modified CTAB method showed the highest yield,while the UniQ ion-exchange method showed the lowest yield of genomic DNA.These three methods gave DNA preparation of comparable homogenecity and efficiency as the template for PCR amplification.Conclusion: The modified CTAB method was better for preparing genomic DNA from Bacillus fastidious.