Immunophenotype of acute leukemia and clinical observation

Objective: To study the immunophenotype and its relationship with prognosis of acute leukemias. Methods: Immunophenotypes were examined by indirect immunofluorescence method in 108 AL. Results: ① In 108 AL, one case of M\-2, one case of M\-5 and one case of L\-3 diagnosed by FAB morphology, finally rediagnosed as MAL and four cases of UAL were diagnosed as M\-0 by immunophenotyping. ②11.5% cases of ALL coexpressed myeloid associated antigens. CD 34 \++ was most frequently expressed in B ALL than T ALL (53%~28%), but no significant difference was found between two groups(P0.05). The marrow blast cells were significantly lower in T ALL than B ALL(P0.05).③ 22 cases (27.8%) of AML coexpressed at least one lymphoid markers. ④ 18 cases expressed CD\-7\++ (22.8%).The early stem cell markers such as CD 34 、 CD 38 、HLA DR were highly expressed in CD\-7\++ AML, CD\-7\++ was rarely expressed in M\-3 but highly expressed in M\-0 M\-1. Conclusion: There was significant value of immunophenotyping for the diagnosis of MAL and M\-0. The expression of CD\-7 in AML might predicts worse prognosis.