An experimental study on the attachment and growth of human skin fibroblast on a porcine collagenic dermal matrix in vitro

Objective: To observe the attachment and growth of fibroblasts on a new porcine collagenic dermal matrix(PCDM) and investigate a method to improve the cell compatibility of the porcine dermal scaffold.Method: The fibroblasts were seeded on two sides of the PCDM.1,7 and 14 days later,the composites were respectively observed by HE staining,scanning electron microscope(SEM)and transmission electron microscope(TEM).Results: Fibroblasts attached better on the papillary dermis than on the reticular dermis in the early period,but more samples of the reticular dermis demonstrated fibroblast stratified structure and infiltration below the cell-seeded surface than the papillary dermis.Conclusion: The new PCDM has good compatibility with human fibroblasts in vitro.It is the most similar to human skin.Modification of the processing technology and removal of the papillary dermis is capable of improving the cell compatibility of the PCDM.