Expression and Purification of VEGF_(121)-KLAK Fusion Protein in E.coli BL21(DE3)

Objective To obtain high-level expression and purification of fusion protein of VEGF121 and amphoteric molecules KLAK in E.coli BL21(DE3) and laid foundations for studying its effection resistence to neoplastic angiogenesis. Methods The genes of VEGF121 and KLAK by RT-PCR method was ligated and was subcloned into pET-28a which was then transformed into competent E.coli BL21(DE3). Induced by IPTG VEGF121-KLAK fusion protein was expressed in recombinant E.coli BL21(DE3) and was detected with SDS-PAGE and Western blot. Results: The genes of VEGF121 and KLAK were obtained and the recombinant expressing vector was constructed. SDS-PAGE and Western blot analysis indicated that the molecular weight of fusion protein was right. Conclusion: The recombinant expressing vector constructed could produce VEGF121-KLAK fusion protein and the high purified fusion protein was obtained.