Nanoparticles dispersing method for toxicology study

Objective To compare the selection of the dispersing agent used and to evaluate the biosafety and to established the optimal dispersing method for nanotoxicological study.Methods The different suspensions of TiO_2 dispersion and cell viability, particle size,Pdl,and absorbance were detected.After comparing their dispersion effect,the best method was selected.Results The results of particle size,polydispersity index ( PdI) ,absorbance,and cell activity showed that 2% Tween 20 dispersion had the smallest particle size.25% FBS dispersion was most stable for the high absorbance,similar to the changes in 2% BSA.2% Tween 20 decrease cell viability.25% FBS and 2% BSA had less effect on cell activity.Conclusions Dispersants suppress the TiO_2 agglomeration. 25% FBS and 2% BSA are better dispersants for the stable dispersion.