The effect of γ-rays radiation pretreatment on enzymatic hydrolysis of corn straw for producing sugar was studied.The relationship between irradiation-dosage and content of reducing sugar was investigated in DNS me-thod.After 1000 kGy irradiation,the content of reducing sugar reached about 317.35%.A synergistic effect between irradiation and enzyme was observed.The reducing sugar yield after enzymatic hydrolysis reached 20.51% when the corn straw powder(0.15 mm) irradiated with a dose of 1 000 kGy.The result shows that the irradiation had significant influence on enzymatic hydrolysis of corn straw.At the 500 kGy pre-irradiation,compared with initial yield,the maximum sugar yield of sample had increased by 13.68% while the irradiated corn straw stored in 20 days.