Preliminary study on the purification methods of human NK cells

Objective:To study the biological characteristics of purified human NK cells Methods:Human NK cells were purified from the peripheral blood by T cell panning, complement lysis and sheep red blood cell rosettes method (SRBC) Compare the cytolytic activity, proliferation ability and IFN γ mRNA expression of the purified NK cells and PBMC by MTT, cell counting and RT PCR respectively Results: NK cells were purified from 10% to 55 73% at most The cytolytic activity and proliferation ability of purified NK cells are stronger than those of PBMC The expression of IFN γ mRNA of the purified NK cells was enhanced when stimulated by IL 2 Conclusion:Purified NK cells have different biological characteristics compared with PBMC or LAK cells