Preparation Optimization of Polyclonal Antibodies Against Three Important Enzymes

OBJECTIVE To prepare high-quanlity polyclonal antibody against chitinase,α-mannosidase and GST. METHODS Corresponding purified proteins with different doses were used to immunize the New Zealand rabbits of different ages. RESULTS When using 0. 3mg·L- 1purified proteins to immunize six-month-old New Zealand rabbit,the polyclonal antibodies possessed higher titer and specificity. CONCLUSION Our results establish a dependable basis for the large-scale production of the related polyclonal antibodies.