蛋黄
卵黄原蛋白
蛋清
卵转铁蛋白
白蛋白
卵清蛋白
血液蛋白质类
生物化学
生物
磷蛋白
蛋白酶
蛋白酵素
化学
蛋白质组
巨球蛋白
酶
免疫学
食品科学
蛋白激酶A
免疫系统
基因
作者
Karlheinz Mann,Matthias Mann
出处
期刊:Proteomics
[Wiley]
日期:2007-12-19
卷期号:8 (1): 178-191
被引量:187
标识
DOI:10.1002/pmic.200700790
摘要
Abstract Using 1‐D SDS‐PAGE, LC‐MS/MS, and MS 3 , we identified 119 proteins from chicken egg yolk, 86 of which were not identified in yolk previously. Proteins were roughly quantitated by calculating their exponentially modified protein abundance index (emPAI) to classify them as major or minor yolk components, and to estimate their distribution between yolk plasma and yolk granular fraction. The proteins with highest abundance were serum albumin, the vitellogenin cleavage products, apovitellenins, IgY, ovalbumin, and 12 kDa serum protein with cross‐reactivity to β2‐microglobulin. In addition yolk contained many other serum and egg white proteins, the proteases nothepsin and thrombin, numerous protease inhibitors, and antioxidative enzymes, such as superoxide dismutase and glutathione peroxidase. Among the moderately abundant proteins were two α2‐macroglobulin‐like proteins different from egg white α2‐macroglobulin, and the major biotin‐binding protein of yolk. An unexpected identification was that of the eggshell matrix protein ovocleidin‐116, which was previously thought to be eggshell‐specific. The list of chicken egg yolk proteins provided in this report is by far the most comprehensive at present and may serve as a starting point for the characterization of less well‐known yolk proteins.
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