成纤维细胞
粘附
整合素
细胞粘附
细胞生物学
佛波
生物
细胞因子
细胞粘附分子
细胞-细胞相互作用
刺激
体外
分子生物学
免疫学
化学
信号转导
细胞
蛋白激酶C
内分泌学
生物化学
有机化学
作者
Albrecht Giuliani,Susanna Spisani,T Cavalletti,Eva Reali,Loredana Melchiorri,Luisa Ferrari,Francesco Lanza,Serena Traniello
标识
DOI:10.1006/cimm.1993.1148
摘要
In our research there was spontaneous adhesion between resting fibroblasts and neutrophils in vitro which could be increased by stimulating either the coculture of cells or each cell type separately with various stimulants. Interferon-γ, interleukin-1, and interleukin-6 significantly increased adhesion; however, the highest adhesive response was obtained when cocultures were treated with phorbol myristate acetate (PMA). As PMA-stimulated fibroblasts show the highest adhesion to resting neutrophils, it was suggested that adhesion was primarily due to an effect on fibroblasts. Without Mg2+ PMA did not stimulate fibroblast adhesion, whereas in the absence of Ca2+ the response was only partially reduced. Spontaneous adhesion was independent of both neutrophil integrins and fibroblast ICAM-1, whereas cytokine-stimulated adhesion was blocked by mAbs against ICAM-1; PMA-stimulated adhesion was not affected by mAbs anti-ICAM-1, but was partially inhibited by mAbs anti-β2 integrins. These results suggested the presence of mechanisms able to modulate the adhesive fibroblast-neutrophil interaction in inflammatory and wound healing processes.
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