遗传学
函数增益
功能(生物学)
细胞生物学
错义突变
癌症研究
点突变
表型
突变
平方毫米
无义突变
基因突变
DNA修复
作者
Dirk P. Dittmer,Sibani Pati,Gerard P. Zambetti,Shelley Chu,Angelika K. Teresky,M Moore,Cathy A. Finlay,Arnold J. Levine
出处
期刊:Nature Genetics
[Springer Nature]
日期:1993-05-01
卷期号:4 (1): 42-46
被引量:782
摘要
We report that the expression of murine or human mutant p53 proteins in cells with no endogenous p53 proteins confers new or additional phenotypes upon these cells. Mutant p53 proteins expressed in cell lines lacking p53 resulted in either enhanced tumorigenic potential in nude mice ((10)3 cells) or enhanced plating efficiency in agar cell culture (human SAOS-2 cells). Also, mutant human p53 alleles, unlike the wild-type p53 protein, could also enhance the expression of a test gene regulated by the multi-drug resistance enhancer-promoter element. These data demonstrate a gain of function associated with p53 mutations in addition to the loss of function shown previously to be associated with mutations in this tumour suppressor gene.
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