活性氧
癌细胞
荧光
癌症
荧光寿命成像显微镜
溶酶体
细胞生物学
癌症研究
生物物理学
材料科学
化学
生物
生物化学
量子力学
物理
遗传学
酶
作者
Hongxing Zhang,Jing Liu,Chenlu Liu,Peng Yu,Minjia Sun,Xiaohan Yan,Jianping Guo,Wei Guo
出处
期刊:Biomaterials
[Elsevier]
日期:2017-07-01
卷期号:133: 60-69
被引量:91
标识
DOI:10.1016/j.biomaterials.2017.04.023
摘要
Lysosomes have recently been regarded as the attractive pharmacological targets for selectively killing of cancer cells via lysosomal cell death (LCD) pathway that is closely associated with reactive oxygen species (ROS). However, the details on the ROS-induced LCD of cancer cells are still poorly understood, partially due to the absence of a lysosome-targetable, robust, and biocompatible imaging tool for ROS. In this work, we brought forward a Si-rhodamine-based fluorescent probe, named PSiR, which could selectively and sensitively image the pathologically more relavent highly reactive oxygen species (hROS: HClO, HO, and ONOO−) in lysosomes of cancer cells. Compared with many of the existing hROS fluorescent probes, its superiorities are mainly embodied in the high stability against autoxidation and photoxidation, near-infrared exitation and emission, fast fluorescence off−on response, and specific lysosomal localization. Its practicality has been demonstrated by the real-time imaging of hROS generation in lysosomes of human non-small-cell lung cancer cells stimulated by anticancer drug β-lapachone. Moreover, the probe was sensitive enough for basal hROS in cancer cells, allowing its further imaging applications to discriminate not only cancer cells from normal cells, but also tumors from healthy tissues. Overall, our results strongly indicated that PSiR is a very promising imaging tool for the studies of ROS-related LCD of cancer cells, screening of new anticancer drugs, and early diagnosis of cancers.
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