活性氧
细胞凋亡
活力测定
膜电位
线粒体
体温过低
化学
细胞生物学
生物物理学
程序性细胞死亡
细胞
纳秒
生理学
生物
生物化学
光学
物理
激光器
作者
Shengyong Yin,Xudong Miao,Xueming Zhang,Xinhua Chen,Hao Wen
摘要
Nanosecond pulsed electric field (nsPEF) is a novel non-thermal tumor ablation technique. However, how nsPEF affect cell physiology at different environmental temperature is still kept unknown. But this issue is of critical clinical practice relevance. This work aim to investigate how nsPEF treated cancer cells react to different environmental temperatures (0, 4, 25, and 37°C). Their cell viability, apoptosis, mitochondrial membrane potential, and reactive oxygen species (ROS) were examined. Lower temperature resulted in higher apoptosis rate, decreased mitochondria membrane potential, and increased ROS levels. Sucrose and N-acetylcysteine (NAC) pre-incubation inhibit ROS generation and increase cell survival, protecting nsPEF-treated cells from low temperature-caused cell death. This work provides an experimental basis for hypothermia and fluid transfusion during nsPEF ablation with anesthesia.
科研通智能强力驱动
Strongly Powered by AbleSci AI