表位
生物
蛋白质组
病毒学
抗体
冠状病毒
表位定位
人口
免疫学
2019年冠状病毒病(COVID-19)
遗传学
医学
环境卫生
病理
传染病(医学专业)
疾病
作者
Anna S. Heffron,Sean McIlwain,Maya F. Amjadi,David Baker,Saniya Khullar,Tammy Armbrust,Peter Halfmann,Yoshihiro Kawaoka,Ajay K. Sethi,Ann C. Palmenberg,Miriam A. Shelef,David H. O’Connor,Irene M. Ong
出处
期刊:PLOS Biology
[Public Library of Science]
日期:2021-06-18
卷期号:19 (6): e3001265-e3001265
被引量:66
标识
DOI:10.1371/journal.pbio.3001265
摘要
The search for potential antibody-based diagnostics, vaccines, and therapeutics for pandemic severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has focused almost exclusively on the spike (S) and nucleocapsid (N) proteins. Coronavirus membrane (M), ORF3a, and ORF8 proteins are humoral immunogens in other coronaviruses (CoVs) but remain largely uninvestigated for SARS-CoV-2. Here, we use ultradense peptide microarray mapping to show that SARS-CoV-2 infection induces robust antibody responses to epitopes throughout the SARS-CoV-2 proteome, particularly in M, in which 1 epitope achieved excellent diagnostic accuracy. We map 79 B cell epitopes throughout the SARS-CoV-2 proteome and demonstrate that antibodies that develop in response to SARS-CoV-2 infection bind homologous peptide sequences in the 6 other known human CoVs. We also confirm reactivity against 4 of our top-ranking epitopes by enzyme-linked immunosorbent assay (ELISA). Illness severity correlated with increased reactivity to 9 SARS-CoV-2 epitopes in S, M, N, and ORF3a in our population. Our results demonstrate previously unknown, highly reactive B cell epitopes throughout the full proteome of SARS-CoV-2 and other CoV proteins.
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