肉眼
融合基因
融合蛋白
辣根过氧化物酶
融合
化学
注意事项
检出限
检测点注意事项
免疫分析
分子生物学
基因
生物
抗体
医学
色谱法
生物化学
病理
遗传学
免疫学
重组DNA
哲学
酶
语言学
作者
Lishuang Yu,Lü Lin,Yuanyuan Yao,Binyong Lin,Wen Xu,Longhua Guo,Wei Xu,Yih‐Bin Lin
标识
DOI:10.1016/j.snb.2021.130473
摘要
The gene fusion of Neurotrophic tropomyosin receptor kinase (NTRK) promotes the formation of tumors and is closely related to multiple cancers. Therefore, the detection of NTRK gene fusion markers has been a research hotspot in recent years. However, up to now, there is still lack of a fast and effective detection method for point-of-care testing (POCT). Herein, a multicolor immunosensor for point-of-care testing NTRK1 gene fusion by the naked eye was fabricated. Au nanobipyramids (Au NBPs) can be etched by 3, 3′, 5, 5′-tetramethylbenzidine ions (TMB2+) to produce a variety of aspect ratios, and the blue shift of its longitudinal plasmon band showed different color changes. So, the Au NBPs were combined with horseradish peroxidase (HRP)-TMB immunoassay system for achieving qualitative and semi-quantitative detection of NTRK1 gene fusion marker within 20 min by the naked eyes. The results shows that the relevant linear range for detection of TRKA protein was 7.5 pg/mL to 240 pg/mL. The constructed immunosensor will be expected to be used to quickly detect NTRK gene fusion markers in remote areas with underdeveloped medical conditions.
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