Residual antler periosteum holds the potential to partially regenerate lost antler tissue

Deer antlers are the only mammalian organs that can fully regenerate, which relies on pedicle periosteum (PP). Interestingly during the growing phase, antlers themselves can regenerate partially lost antler tissue. However, what tissue type in the growing antlers fulfills this role is not known. Following antler removal during the growing phase, a "second" antler regenerates from the stump. In this study, the "second" antler growing from the cut antler base (AB) was examined in both red and sika deer. The results showed that all regenerating antlers were formed from the peripheral edge of the AB, where the antler periosteum (AnP) is located. The growth center showed a clear demarcation from the AB bone in red deer. Therefore, it is highly likely that AnP is the tissue that possesses the potential. Factors that might affect this potential were explored and the main factor was found to be AB calcification, which was controlled by rising androgens. Thus, the ultimate antler regeneration potential of the AnP was assessed through castration and repeated antler removal. The results demonstrated that the regeneration potential of AnP was somewhat limited and inferior to that of the PP. The ability of AnP to achieve partial regeneration may be evolutionarily conserved, as the regeneration of partially lost antlers within the season is secured; whereas, with PP, a new set of antlers in the next season is guaranteed. This two-level mechanism may signify how evolutionarily important it is for deer to possess reasonably intact antlers.