The research work was conducted to determine the production of mycotoxins from Alternaria alternata isolated from carob leaves by the thin layer chromatography technique. The results showed that the production of Altertoxin I, and Alternariol monomethyl ether by A. alternata (A1) and of Alternariol monomethyl ether by A. alternata (A2), while A. alternata (A4) produced Altertoxin I. A. alternata (A3) did not produce any mycotoxins.