差异凝胶电泳
凝胶电泳
聚丙烯酰胺凝胶电泳
色谱法
荧光
电泳
化学
二维凝胶电泳
颜色标记
显著性差异
蛋白质凝胶电泳
分子量大小标记
核酸凝胶电泳
分子生物学
生物
生物化学
蛋白质组学
基因
统计
物理
量子力学
酶
数学
作者
Mustafa Ünlü,Mary E. Morgan,Jonathan S. Minden
出处
期刊:Electrophoresis
[Wiley]
日期:1997-01-01
卷期号:18 (11): 2071-2077
被引量:2088
标识
DOI:10.1002/elps.1150181133
摘要
We describe a modification of two-dimensional (2-D) polyacrylamide gel electrophoresis that requires only a single gel to reproducibly detect differences between two protein samples. This was accomplished by fluorescently tagging the two samples with two different dyes, running them on the same 2-D gel, post-run fluorescence imaging of the gel into two images, and superimposing the images. The amine reactive dyes were designed to insure that proteins common to both samples have the same relative mobility regardless of the dye used to tag them. Thus, this technique, called difference gel electrophoresis (DIGE), circumvents the need to compare several 2-D gels. DIGE is reproducible, sensitive, and can detect an exogenous difference between two Drosophila embryo extracts at nanogram levels. Moreover, an inducible protein from E. coli was detected after 15 min of induction and identified using DIGE preparatively.
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