化学
荧光
单糖
色谱法
发色团
果糖
生物化学
有机化学
物理
量子力学
作者
Mariia Pushina,Andrej Penavic,Sepideh Farshbaf,Pavel Anzenbacher
出处
期刊:ACS Sensors
[American Chemical Society]
日期:2021-10-20
卷期号:6 (11): 4001-4008
被引量:24
标识
DOI:10.1021/acssensors.1c01371
摘要
Accurate monitoring of sugar levels is essential for many fields from food industry to human health. Here, we developed FRET-based dual chromophore sensors for saccharides that form oxazolidine boronate and may be employed as a noninvasive method for monitoring of sugar levels in biological fluids, namely, urine. The saccharide-binding properties of the sensors were studied using fluorescence spectroscopy and utilized in the determination of saccharides in a high-throughput manner. Here, two fluorescent sensors were successful in the classification of nine different monosaccharides and disaccharides with 100% correct classification. Furthermore, the dual chromophore self-assembled sensors were successfully utilized for the quantitative determination of important carbohydrates such as glucose in the presence of competitive saccharides (fructose) and in complex media (urine) without sample pretreatment. The present fluorescent sensors allow for quantification of glucose in a concentration range of 0-60 mM, which matches the concentration range of frequently used urinalysis test strips.
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