转录组
生物
微泡
癌变
腺癌
小RNA
蛋白质组
干细胞
癌症干细胞
癌症研究
计算生物学
细胞生物学
癌症
生物信息学
基因
基因表达
遗传学
作者
Haitao Luo,Yuanyuan Zheng,Jun Tang,Lijuan Shao,Yiheng Mao,Wei Yang,Xiaofei Yang,Li Yang,Ruijun Tian,Furong Li
标识
DOI:10.1038/s41525-021-00217-5
摘要
Abstract Lung adenocarcinoma is heterogeneous and hierarchically organized, with a subpopulation of stem-like cells (CSCs) that reside at the apex of the hierarchy, in which exosomes act as important mediators by transporting specific molecules among different cell populations. Although there have been numerous studies on tumor exosomes, the constituents and functional properties of CSC-derived exosomes are still poorly characterized. Here we present a detail transcriptome and proteome atlas of the exosomes released by human lung adenocarcinoma stem-like cells (LSLCs). The transcriptome analysis indicates the specific patterns of exosomal constituents, including the fragmentation of transcripts and the low-level presence of circular RNAs, and identifies multiple exosomal-enriched mRNAs and lncRNAs. Integrative analysis of transcriptome and proteome data reveals the diverse functions of exosomal-enriched RNAs and proteins, many of which are associated with tumorigenesis. Importantly, several LSLC markers we identified are highly expressed in LSLC-derived exosomes and associate with poor survival, which may serve as promising liquid biopsy biomarkers for lung adenocarcinoma diagnosis. Our study provides a resource for the future elucidation of the functions of tumor-derived exosomes and their regulatory mechanisms in mediating lung cancer development.
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