Enzyme Cascade Reaction‐Propelled Multicompartmental Colloidal Motors

Compartmentalization is a crucial natural methodology to enable multiple biocatalytic transformations to proceed efficiently. Herein, we report a biocompatible multicompartmental colloidal motor that can achieve autonomous movement in the biological environment through two-enzyme cascade reactions of immobilized enzymes. The colloidal motors with the heterogeneous multicompartment structure were prepared in one step by microfluidic technology, and the compartmentalized encapsulation of glucose oxidase (GOD) and catalase (CAT) was realized. The fabricated colloidal motor was size controllable by tuning the flow rates of the microfluidic system, and its autonomous movement can be triggered by good responsiveness to the alkaline environment. In glucose medium of pH 7.5, the pH-responsive alginate cores of the colloidal motor swell to facilitate fuel penetration and enzyme-catalyzed reactions. The enzyme cascade between GOD and CAT immobilized in the colloidal motor chamber results in the self-propulsion of the colloid motor in glucose medium. The compartmentalized encapsulation of immobilized enzyme improves the stability of the enzyme and enables multicompartmental colloidal motors to self-propel in an alkaline intestinal environment through an enzyme cascade reaction. These features indicate that such multicompartmental colloidal motors actuated by enzyme cascade reaction in biocompatible fuel have great potential for co-encapsulation and autonomous movement in different applications.