Proteomics analysis of the hypothalamus of high-fat diet fed mice after Lactiplantibacillus plantarum Y44 administration

Probiotics could regulate human health via changing gut microbiota. Our previous studies found that Lactiplantibacillus plantarum Y44 (L. plantarum Y44) was shown to reverse obesity-related symptoms of high-fat diet fed mice by influencing gut microbiota, which ameliorated lipid metabolism disorders and intestinal inflammation. In this study, the effects of L. plantarum Y44 on high-fat fed mice and the involved mechanisms were investigated through hypothalamus proteomics analysis, which revealed 22 downregulated and 21 upregulated proteins. Differentially expressed proteins between L. plantarum Y44 intervention group and high-fat fed group in hypothalamus of mice involved in metabolic pathways were concentrated in endocrine regulation of carbohydrate, amino acid and energy generation pathways. The hypothalamus proteomics results about the influences on most of differentially expressed proteins of L. plantarum Y44 administering high-fat fed mice has been verified. Therefore, L. plantarum Y44 would be a potential functional strain for the amelioration of high-fat diet associated health problems.