阿卡波糖
检出限
淀粉酶
化学
色谱法
支链淀粉
水溶液
急性胰腺炎
生物化学
医学
内科学
酶
直链淀粉
淀粉
有机化学
作者
Mei Zhao,Limei Luo,Yongxian Guo,Binglu Zhao,Xiangfeng Chen,Xingang Shi,Mashooq Khan,Jin‐Ming Lin,Qiongzheng Hu
出处
期刊:ACS Sensors
[American Chemical Society]
日期:2022-01-20
卷期号:7 (2): 593-600
被引量:12
标识
DOI:10.1021/acssensors.1c02489
摘要
α-Amylase (AMS) in human serum is a critical biomarker for the early diagnosis of pancreatic damage. In addition, the inhibition of α-amylase has long been thought to decrease the occurrence of diabetes. Thus, it is critical to construct a facile and convenient method for the determination of AMS and its inhibitor. In this study, we demonstrate a novel amylase sensor based on translating the viscosity change of the aqueous solution into the difference of the water diffusion length on a pH paper strip. AMS can be quantitatively detected by measuring the viscosity change of the amylopectin solution in the presence of AMS with different concentrations. The paper-based AMS sensor has a very high sensitivity with a detection limit of 0.017 U/mL and also shows excellent specificity. In addition, the inhibitory effect of acarbose on AMS is demonstrated with the IC50 value determined to be 21.66 ± 1.13 μg/mL. Furthermore, it is also evaluated for the detection of AMS in human serum samples of healthy people and acute pancreatitis patients. The difference in amylase levels between the two groups is unambiguously distinguished. Overall, this study provides a very simple, cost-effective, equipment-free, high-throughput, and label-free method for rapid and quantitative detection of α-amylase and may have significant applications in the diagnosis of acute pancreatitis and the screening of AMS inhibitors.
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