Exogenous gibberellin induced regreening through the regulation of chlorophyll and carotenoid metabolism in Valencia oranges

In the present study, we studied the effects of gibberellic acid (GA) on chlorophyll and carotenoid metabolites and related gene expression during the regreening process in Valencia orange fruits (Citrus sinensis Osbeck). During the regreening, fruits treated with GA turned green much faster than those of the control. Compared with untreated fruits, chlorophyll accumulation was induced and the content of carotenoids (β-cryptoxanthin, all-trans-violaxanthin, and 9-cis-violaxanthin) was decreased by the GA treatment. Chlorophyll and carotenoid contents following GA treatment appeared to be highly regulated at the gene transcription level. Correspondingly, the up-regulation of chlorophyll biosynthesis genes (CitGGDR, CitCHL27, CitPORA, and CitCAO) and down-regulation of degradation genes (CitCLH1, CitSGR, CitPPH, CitPAO, and CitRCCR) led to the increase of chlorophyll contents, and the down-regulation of carotenoid biosynthesis genes (CitPSY, CitPDS, CitZDS, CitLCYb2, and CitHYb) led to the decrease of carotenoid contents. These observations indicated that GA acted as a crucial regulator in the regreening process of citrus fruits.