Abstract 889: Mouse model for chemotherapy-induced alopecia with transplantation of human hair follicles onto immune deficient mouse

Abstract Optimized research models of chemotherapy-induced alopecia (CIA) require for further understanding the pathogenesis and protection of CIA. The present study was aimed to develop a new mouse model for CIA by follicular unit transplantation of human hair follicles onto the immune-deficient mice. At 22 weeks after transplantation, single dose of cyclophosphamide (Cph) was treated in mice of Cph100 (100mg/kg) and Cph150 (150mg/kg) groups. On day 6 of treatment, hair follicles showed dystrophic anagen representing with swollen dermal papilla and ectopic melanin clumping in the hair bulb. In addition, up-regulated P53 expression and apoptotic matrix keratinocytes, and down-regulated expression of Ki67 and melanogenic protein in the hair bulb were also noted in both groupsin Cph100 and Cph150. Subsequently, hair follicles in Cph100 appeared to recover as anagen hair follicles after 9 days. Ki67 and melanogenic protein expression returned as the levels analogous to the control. On the other hands, hair follicles of Cph150 prematurely entered dystrophic catagen-telogen phase after 9 days of treatment. Ki67 and melanogenic protein expression in immunofluorescence staining were barely visible. Those findings indicate that this model represented two pathways of dystrophic anagen pathway in Cph100 and dystrophic catagen pathway in Cph150. Our model will be a useful tool for the further understanding of the impact of chemotherapy on the human hair follicle. Citation Format: Ohsang Kwon, Ji-Seon Yoon, Mira Choi, Chang Yup Shin, Kyu Han Kim. Mouse model for chemotherapy-induced alopecia with transplantation of human hair follicles onto immune deficient mouse. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 889. doi:10.1158/1538-7445.AM2015-889