外体
微泡
电穿孔
体内
内吞作用
细胞生物学
体外
基因传递
转染
药物输送
生物
化学
生物化学
基因
小RNA
细胞
生物技术
有机化学
作者
Zhaogang Yang,Jing Xie,Jing Zhu,Kang Chen,Chi‐Ling Chiang,Xinmei Wang,Xiaobing Wang,Tairong Kuang,Feng Chen,C. Zhou,Aili Zhang,Bo Yu,Robert J. Lee,Lesheng Teng,L. James Lee
标识
DOI:10.1016/j.jconrel.2016.10.008
摘要
Exosomes, the smallest subgroup of extracellular vesicles, have been recognized as extracellular organelles that contain genetic and proteomic information for long distance intercellular communication. Exosome-based drug delivery is currently a subject of intensive research. Here, we report a novel strategy to produce nanoscale exosome-mimics (EMs) in sufficient quantity for gene delivery in cancer both in vitro and in vivo. Size-controllable EMs were generated at a high yield by serial extrusion of non-tumorigenic epithelial MCF-10A cells through filters with different pore sizes. siRNA was then encapsulated into the EMs by electroporation. Biosafety and uptake efficiency of the EMs were evaluated both in vitro and in vivo. The mechanism underlying their cellular endocytosis was also studied.
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