Pancreatic lipase inhibition of strictinin isolated from Pu’er tea (Cammelia sinensis) and its anti-obesity effects in C57BL6 mice

Abstract Strictinin isolated from Pu’er tea was demonstrated to inhibit pancreatic lipase in vitro. In an olive oil tolerance test, the administration of strictinin (100 mg/kg) significantly reduced the blood triglyceride level and increased the fecal oil content of C57BL6 mice. In a long-term animal study, the body weight gained via high-fat diet was significantly reduced in the mice supplemented with strictinin of 130 mg/kg. Further analysis showed that weight of epididymal fat, but not that of liver or muscle, increased in mice fed with high-fat diet, and the increase of fat was reduced in the mice supplemented with strictinin. Correspondingly, sizes of adipocytes in epididymis were substantially enlarged in mice fed with high-fat diet, and the enlargement of adipocytes was decreased when strictinin was supplemented. Levels of blood triglyceride, cholesterol and glucose were elevated in mice fed with high-fat diet, and these elevated levels were reduced when strictinin was supplemented.