鱼精蛋白
纳米团簇
化学
纳米化学
荧光
石墨烯
双金属片
Zeta电位
材料科学
氧化物
纳米颗粒
铜
胰蛋白酶
肝素
核化学
纳米技术
有机化学
金属
生物化学
酶
物理
量子力学
作者
Jinlan Yang,Naizhong Song,Qiong Jia
标识
DOI:10.1007/s00604-018-3179-6
摘要
An electrostatically controlled fluorometric assay is described that is based on the use of silver/copper bimetallic nanoclusters. The nanoclusters were coated with polyethyleneimine (PEI-Ag/CuNCs). At pH 7.4, these particles are positively charged. Their blue fluorescence (with excitation/emission peaks at 341/464 nm) depends on local pH values and temperature. If graphene oxide (which is negatively charged at pH 7.4) is introduced, the fluorescence of the PEI-Ag/CuNCs is quenched. Based on various electrostatic interactions, three kinds of biomacromolecules were detected by fluorometry. These include (negatively charged) heparin, (positively charged) protamine, and (virtually uncharged) trypsin. Heparin is detected by using GO/PEI-Ag/CuNCs, protamine by using GO/heparin/PEI-Ag/CuNCs, and trypsin by using GO/protamine/heparin/PEI-Ag/CuNC. The detection limits and linear ranges are 4.8 nM and 10–450 nM for heparin, 0.09 μg·mL−1 and 0.25–5 μg·mL−1 for protamine, and 0.03 μg·mL−1 and 0.05–1 μg·mL−1 for trypsin. Zeta potentials of the various substances in the system were determined to elucidate the detection mechanism. Comceivably, the method provides a widely applicable approach for electrostatically controlled biomolecular assays.
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