DNA
底漆(化妆品)
滚动圆复制
纳米技术
DNA聚合酶
序列(生物学)
DNA纳米技术
计算机科学
计算生物学
生物
分子生物学
遗传学
物理
材料科学
热力学
作者
Liying Wang,Kaiyun Song,Yuanyuan Qu,Yangyang Chang,Zhongping Li,Chuan Dong,Meng Liu,John D. Brennan,Yingfu Li
标识
DOI:10.1002/anie.202010693
摘要
Abstract φ 29 DNA polymerase (Pol φ 29) is capable of synthesizing long‐chain single‐stranded (ss) DNA molecules by copying the sequence of a small ss circular DNA template (ssCDT) in a process known as rolling circle amplification (RCA). The use of a ssCDT in RCA, however, comes with a key drawback: the rate of DNA synthesis is significantly reduced. We hypothesize that this issue can be overcome using a very long linear ssDNA template with a repeating sequence. To test this idea, we engineered a DNA assembly, which we denote “micrometer‐sized DNA track” (μDT). This μDT, with an average length of ≈13.5 μm, is made of a long chain DNA with a primer‐binding domain at its 3′ end and ≈1000 repeating sequence units at its 5′ end, each carrying a DNA anchor. We find that Pol φ 29 copies μDT at a speed ≈5‐time faster than it does a related ssCDT. We use this to design a simple all‐in‐one printed paper device for rapid and sensitive detection of microRNA let‐7. This paper sensor is capable of detecting 1 pM let‐7a in 10 minutes.
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