Spatial and temporal diversity of glycome expression in mammalian brain

Significance Deciphering molecular mechanisms at the glycome level in mammalian brain remains a missing piece of the puzzle in molecular neuroscience due to the intrinsic complexity of glycosylation and the lack of analytical tools. Here, we uncovered the variation and diversity of glycome expression in human and mouse brain samples according to spatial and temporal differences. We further constructed a comprehensive synthesis map using glycans structurally elucidated by LC-MS/MS and found strong evidence on the conservation and developmental divergence of human and mouse prefrontal cortex N-glycome. Our data could be the reference for future mammalian brain glycome study. Furthermore, it provides valuable information on human brain glycome, which has languished in relative obscurity.