内体
磷脂酰肌醇
效应器
拟南芥
液泡蛋白分选
细胞生物学
液泡
激酶
化学
生物
生物化学
基因
细胞质
细胞内
突变体
作者
Han Nim Lee,Hyera Jung,Taijoon Chung
出处
期刊:Methods in molecular biology
日期:2020-01-01
卷期号:: 109-118
被引量:1
标识
DOI:10.1007/978-1-0716-0767-1_10
摘要
Phosphatidylinositol-3-phosphate (PI3P) is a signaling phospholipid enriched in the membranes of late endosomes (LE) and vacuoles. PI3P mediates vacuolar and endosomal trafficking through recruiting PI3P-binding effector proteins to the LE. PI3P is produced from phosphatidylinositol by the PI 3-kinase complex containing VACUOLAR PROTEIN SORTING 34 (VPS34). The role of PI3P has been elucidated by using genetically encoded PI3P biosensors. We previously showed that Arabidopsis VPS38, a component of the VPS34 complex, localized at the LE and that VPS38 is essential for proper PI3P distribution in endosomal and vacuolar trafficking routes. In this chapter, we describe methods for microscopic imaging of PI3P using the PI3P biosensor citrine-2 × FYVE and the PI 3-kinase inhibitors.
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