Abstract A new Light‐Up fluorescent probe ( FA‐P ) for detection of formaldehyde was designed and synthesized based on the donor‐excited photo induced electron transfer ( d ‐PeT) process. The probe FA‐P was constructed by integrating a benzothiazole derivatives as the fluorescent skeleton and homoallylamino group as the specific recognition site. It showed the unique advantages of easy‐preparation, and excellent selectivity response toward formaldehyde. Besides, The probe FA‐P can not only detect formaldehyde gas through the prepared test paper, but also detect formaldehyde in serum solution.