点击化学
叠氮化物
炔烃
寡核苷酸
组合化学
化学
有机化学
催化作用
生物化学
DNA
作者
Afaf H. El‐Sagheer,Tom Brown
标识
DOI:10.1002/0471142700.nc0433s35
摘要
The Cu(I)-catalyzed alkyne-azide cycloaddition (CuAAC) reaction has been used to synthesize cyclic mini-DNA duplexes. The reaction is carried out on 5'-alkyne-3'-azide-labeled hairpin loop oligonucleotides and proceeds in high yield under mild conditions in as little as 5 min. The resultant duplexes have very high thermal stability and their CD spectra are characteristic of normal B-DNA.
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