Metarhizium anisopliae and Beauveria bassiana had been used to control Dendrolimus punctatus in lab .The lab test showed that there were not much differences in the toxicity to 3rd ~ 4th instar larvae of D . punctatus between M .anisopliae and B .bassiana at 25 ℃.The median lethal concentration of M .anisopliae was 6.38 108 sporesL-1 , and the median lethal time was 8.60 ~ 15.19 days for the concentration of 1.0 1011 ~ 1.0 107 sporesL-1 .The median lethal concentration of B .bassiana was 5.06 108 sporesL-1 , and the median lethal timewas 7.80 ~ 14.79 days for the concentration of 1.0 1011 ~ 1.0 107 sporesL-1 .The strain of M .anisopliae was more tolerant to high temperature and drought than B .bassiana .At high temperature or low humidity , the pathogenicity on D .punctatus was superior to that by B .bassiana .The lab test indicated that M . anisopliae was of greater value in controlling D .punctatus .