Spin Trapping of the Nitrogen-centered Radicals. Characterization of the DMPO/DEPMPO Spin Adducts

Abstract To develop the spin trapping utility for the detection of N-centered radicals generated in biological systems, 5,5′-dimethylpyrroline-1-oxide (DMPO) and 5-diethoxyphosphoryl-5-methyl-1-pyrroline-N-oxide (DEPMPO), have been applied for the detection of chemically generated N-centered radicals in aqueous solution. Both spin traps yielded N-radical adducts, which were characterized by the EPR parameters.