二硫键
化学
抗体
重组DNA
肽
组合化学
生物化学
生物
免疫学
基因
作者
Sheng Gu,Dingyi Wen,Paul H. Weinreb,Yaping Sun,Lihe Zhang,Susan F. Foley,Rashmi Kshirsagar,David R. Evans,Sha Mi,Werner Meier,R. Blake Pepinsky
标识
DOI:10.1016/j.ab.2010.01.019
摘要
Trisulfides are a posttranslational modification formed by the insertion of a sulfur atom into a disulfide bond. Although reports for trisulfides in proteins are limited, we find that they are a common modification in natural and recombinant antibodies of all immunoglobulin G (IgG) subtypes. Trisulfides were detected only in interchain linkages and were predominantly in the light-heavy linkages. Factors that lead to trisulfide formation and elimination and their impact on activity and stability were investigated. The peptide mapping methods developed for characterization and quantification of trisulfides should be applicable to any antibody and can be easily adapted for other types of proteins.
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