中心组合设计
响应面法
硫酸铵
大肠杆菌
酵母抽提物
发酵
化学
食品科学
生物化学
重组DNA
分式析因设计
色谱法
析因实验
生化工程
数学
统计
基因
工程类
作者
Jie Zhao,Yonghong Wang,Ju Chu,Siliang Zhang,Yingping Zhuang,Zhongyi Yuan
标识
DOI:10.1007/s10295-007-0301-x
摘要
Pyruvate oxidase (PyOD) is a very useful enzyme for clinical diagnostic applications and environmental monitor. Optimization of the fermentation medium for maximization of PyOD constitutively, production by Escherichia coli DH5alpha/pSMLPyOD was carried out. Response surface methodology (RSM) was used to optimize the medium constituents. A 2(6-2) fractional factorial design (first order model) was carried out to identify the significant effect of medium components towards PyOD production. Statistical analysis of results shows that yeast extract, ammonium sulfate and composite phosphate were significant factors on PyOD production. The optimized values of these three factors were obtained by RSM based on the result of a 2(3) central composite rotatable design. Under these proposed optimized medium, the model predicted a PyOD activity of 610 U/L and via experimental rechecking the model, an activity of 670 U/L was attained.
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