蓝藻
基因组
生物
计算生物学
遗传学
基因
细菌
作者
Annesha Sengupta,Anindita Bandyopadhyay,Debolina Sarkar,John I. Hendry,Max G. Schubert,Deng Liu,George M. Church,Costas D. Maranas,Himadri B. Pakrasi
出处
期刊:MBio
[American Society for Microbiology]
日期:2024-02-15
标识
DOI:10.1128/mbio.03530-23
摘要
Genome streamlining is an evolutionary strategy used by natural living systems to dispense unnecessary genes from their genome as a mechanism to adapt and evolve. While this strategy has been successfully borrowed to develop synthetic heterotrophic microbial systems with desired phenotype, it has not been extensively explored in photoautotrophs. Genome streamlining strategy incorporates both computational predictions to identify the dispensable regions and experimental validation using genome-editing tool, and in this study, we have employed a modified strategy with the goal to minimize the genome size to an extent that allows optimal cellular fitness under specified conditions. Our strategy has explored a novel genome-editing tool in photoautotrophs, which, unlike other existing tools, enables large, spontaneous optimal deletions from the genome. Our findings demonstrate the effectiveness of this modified strategy in obtaining strains with streamlined genome, exhibiting improved fitness and productivity.
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