Electrochemical Biosensing of Myeloperoxidase in Undiluted Serum on Screen Printed Electrodes Using PEG Coated Hierarchical Gold Nanostructures

We present a novel electrochemical biosensor for myeloperoxidase (MPO), a cardiovascular disease biomarker, using gold screen printed electrodes with hierarchical gold nanostructures formed through in situ electrodeposition process. Incorporation of polyethylene glycol (PEG) enables immobilization of desired antibodies for target-specific biosensing and offers high sensitivity and excellent anti-fouling capability when working with complex samples such as serum. The utility of this sensor is demonstrated by detection of clinically relevant MPO concentrations in diluted and undiluted serum samples.