Combination of atmospheric and room temperature plasma and ribosome engineering techniques to enhance the antifungal activity of Bacillus megateriumL2 against Sclerotium rolfsii

Sclerotium rolfsii is an extremely destructive phytopathogenic fungus that causes significant economic losses. Biocontrol strategies utilizing antagonistic microorganisms present a promising alternative for controlling plant pathogens. Bacillus megaterium L2 has been identified as a potential microbial biocontrol agent in our previous study; however, its efficacy in controlling pathogens has yet to meet current demands. This study aims to enhance the antifungal activity of strain L2 against S. rolfsii R-67 through a two-round mutagenesis strategy and to preliminarily investigate the mutagenesis mechanism of the high antifungal activity mutant.