An Oxygen-scavenging System without Impact on DNA Mechanical Properties in Single-Molecule Fluorescence Experiments

Oxygen scavenging systems (OSSs) are critical for dye stability in single-molecule fluorescence (SMF) experiments. However, the commonly used protocatechuic acid (PCA)/protocatechuate-3,4-dioxygenase (PCD) OSS alters DNA mechanical properties, limiting its applicability....