化学
显微镜
电化学
扫描电化学显微镜
循环伏安法
膜
电化学电池
分辨率(逻辑)
纳米技术
纳米尺度
分析化学(期刊)
生物物理学
材料科学
电极
生物化学
色谱法
光学
物理
物理化学
人工智能
计算机科学
生物
作者
Rong Jin,Wei Zhou,Yanyan Xu,Dechen Jiang,Daoyuan Fang
标识
DOI:10.1021/acs.analchem.3c00114
摘要
The electrochemical visualization of proteins in the plasma membrane of single fixed cells was achieved with a spatial resolution of 160 nm using scanning electrochemical cell microscopy. The model protein, the carcinoembryonic antigen (CEA), is linked with a ruthenium complex (Ru(bpy)32+)-tagged antibody, which exhibits redox peaks in its cyclic voltammetry curves after a nanopipette tip contacts the cellular membrane. Based on the potential-resolved oxidation or reduction currents, an uneven distribution of membrane CEAs on the cells is electrochemically visualized, which could only be achieved previously using super-resolution optical microscopy. Compared with current electrochemical microscopy, the single-cell scanning electrochemical cell microscopy (SECCM) strategy not only improves the spatial resolution but also utilizes the potential-resolved current from the antibody–antigen complex to increase electrochemical imaging accuracy. Eventually, the electrochemical visualization of cellular proteins at the nanoscale enables the super-resolution study of cells to provide more biological information.
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