检测点注意事项
纳米技术
检出限
纳米棒
化学
荧光
显色的
干扰(通信)
材料科学
色谱法
计算机科学
电信
光学
物理
频道(广播)
免疫学
生物
作者
Yuanyuan Cao,Tong Bu,Haiyu Wu,Xiaobin Jia,Ying Wang,Cuijuan Xuan,Pei Jia,Bing Zheng,Jun Zhao,Yuting Zhuang,Li Wang
标识
DOI:10.1021/acs.analchem.3c02718
摘要
Nanomaterials-based immunochromatographic assays (ICAs) are of great significance in point-of-care testing (POCT), yet it remains challenging to explore low background platforms and high chromogenic intensity probes to improve detection performance. Herein, we reported a low interference and high signal-to-noise ratio fluorescent ICA platform based on ultrabright persistent luminescent nanoparticles (PLNPs) Zn2GeO4: Mn, which could produce intense photoluminescence at 254 nm excitation to reduce background interference from ICA substrates and samples. The prepared immunosensor was successfully applied in T-2 toxin detection with a remarkable limit of detection of 0.025 ng/mL, which was 22-fold more sensitive compared with that of traditional gold nanoparticles. Ultimately, a portable 3D-printed detection device equipped with a smartphone analyzing application was fabricated for quantitative readout in POCT, achieving favorable recoveries in practical sample detection. This work provides a creative attempt for ultrabright PLNP-based low background ICA, and it also guarantees its feasibility in practical POCT.
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