Neopusillimonas aromaticivorans sp. nov. isolated from poultry manure

A polyphasic taxonomic approach was used to characterize a novel bacterium, designated strain CC-YST667 T , isolated from poultry manure sampled in Taiwan. The cells were observed to be aerobic, motile and non-spore-forming rods, displaying positive reactions for oxidase. Optimal growth of CC-YST667 T was observed at 25 °C, pH 8.0 and with 1 % (w/v) NaCl. The polar lipid profile consisted of phosphatidylmonomethylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and multiple unidentified polar lipids. The major polyamine was spermidine. The major cellular fatty acids (>5 %) included C 16 : 0 , C 17 : 0 cyclo, C 19 : 0 cyclo ω8 c and C 14 : 0 3OH/iso-C 16 : 1 I. On the basis of the results of analysis of 16S rRNA gene sequences, this isolate showed the closest phylogenetic relationship with ‘ Neopusillimonas minor’ (with 98.2 % similarity) and Paralcaligenes ureilyticus (with 97.3 % similarity) of the family Alcaligenaceae . The draft genome, (3.3 Mb) with a DNA G+C content of 57.2 mol%, harboured various genes involved in the biodegradation of aromatic hydrocarbons. CC-YST667 T shared highest orthologous average nucleotide identity (OrthoANI) with the type strains of species of of the genera Neopusillimonas (72.4‒77.9 %, n =2), Pusillimonas (72.8‒73.0 %, n =2) and Pollutimonas (71.7‒73.0 %, n =5). On the basis of its distinct phylogenetic, phenotypic and chemotaxonomic traits together with the results of comparative 16S rRNA gene sequencing, OrthoANI, digital DNA–DNA hybridization (DDH) and the phylogenomic placement, strain CC-YST667 T is considered to represent a novel species of the genus Neopusillimonas , for which the name Neopusillimonas aromaticivorans sp. nov. is proposed. The type strain is CC-YST667 T (=BCRC 81321 T =JCM 34761 T ).