High performance liquid chromatography based determination of 6-gingerol,8-gingerol and 10-gingerol in seed and tender ginger

We employed high performance liquid chromatography(HPLC) to determine the contents of 6-,8-,and 10-gingerol in seed and tender ginger.The determination conditions were an Inertsil ODS-SP column(250 mm×4.6 mm,5 μm),a binary eluent under gradient condition and ultraviolet detection at 280 nm wavelength.Experimental results show that the contents of 6-,8-and 10-gingerol in seed ginger are separatively two times,one and a half times and two times of those in tender ginger.