Improving peroxidase-like activity of hemoglobin by recombining hemoglobin using chemically modified heme

The phenol or 4-biphenylol was used to modify the heme.The modified hemin group was recombined with apo-hemoglobin to prepare novel artificial hemoglobin.Absorption spectral scan demonstrated that the artificial recombined hemoglobins with modified heme were obtained.Experimental results showed that the peroxidase-like activity of modified hemoglobins was enhanced compared with that of native hemoglobin.And the enzymatic activity of modified hemoglobin with 4-biphenylol modified heme was as about 1.6 folds than that of the native hemoglobin.